Part:BBa_K3912001
Template 2
Template 1 and 2 makes a molecular switch together to reflect whether the tau protein exists in the reaction system. The reaction that takes place is Recombinase Polymerase Amplification(RPA), in which we use two primers, called Amplification F and Amplification R. Template 2 is the single-strand DNA that make a loop-stem structure with the Template 1 strand. It contains the tau-aptamer and the loop. The loop contains the sequence of the Amplification R, called Amp-R, while the aptamer pairs part with Template 1 at its 5’ end and part with the left sequence of Template 2. And other sequence of Template 2 pairs with Template 1. In the RPA reaction, when tau protein is absent, no reaction will start; but when tau is present, the protein will bind with the aptamer sequence and pull it away, turning the loop-stem structure into a linear structure, and Amp-R will act as the template to make a double-strand DNA molecule with Template 1 strand as the primer. And then the primer-binding sequence replicated from Amp-R will exist on the prolonged Template 1 strand for the Amplificaion R to make a prolonged Template 2 strand, with the binding sequence for Amplification F on it (also the probe-binding site), thus the complete double-strand DNA molecule is formed and the RPA reaction will continue.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal NotI site found at 7 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16 - 1000COMPATIBLE WITH RFC[1000]
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